FIG. 7.

Effect of Mn supplementation on the expression of MiGM3′ at the RNA level. Nitrogen-limited cultures of T-5 were grown in the presence and absence of Mn. RNA was extracted, separated by electrophoresis, transferred to a membrane, and probed as described in the text. (A) Northern blots probed with the gpd gene; (B) Northern blots probed with the mnp1 gene, showing RNA from endogenous genes; (C) Northern blots probed with the gfp gene. Lanes 1 and 2 contain RNA from transformant T-1 carrying the control plasmid pUB; lanes 3 and 4 contain RNA from transformant T-5 carrying pUMiGM3′; lanes 2 and 4 contain RNA from cells growing in the presence of 180 μM Mn; lanes 1 and 3 contain RNA from cells grown in the absence of exogenous Mn.