Figure 4.

EZH2 is downregulated in the thymus after three weeks spent at 2× g and regulates H3K27 methylation in thymocytes. (A,B) Expression of PRC2 components (EZH2, Suz12 and EED) studied by Western blotting. (A) Relative expression of EZH2, Suz12 and EED normalized to GAPDH. (B) Representative Western blots obtained with four mice in each group. Arrow indicates Suz12 protein. (C,D) To evaluate the contribution of EZH2 activity to the H3K27me3 level in the thymus, thymocytes were exposed, or not, to the specific inhibitor GSK126 at a concentration of 2 µM for 10 h, and histones were extracted to perform Western blots. Here, ‘t0’ indicates H3K27me3 level in thymic cells before treatment. (C) Histogram presenting the relative expression of H3K27me3 normalized to H3. (D) Western blot representative of three independent experiments. Data are mean ± SD of three independent experiments. ANOVA was used to reveal statistically significant differences. * p < 0.05.