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. 2022 Jun 27;23(13):7133. doi: 10.3390/ijms23137133

Figure 5.

Figure 5

Hypergravity induces H3K27 tri-methylation increase at Vβ regions in the SCIET27 cell line. (A) Schematic representation of primer positions along one Vβ gene segment. Vβ12-1, Vβ13-1, Vβ13-3 and Vβ14 segments are composed of two exons (gray). Recombination signal sequences (RSS) are localized at the 3′ end of the V exon (orange triangle). Two pairs of primers were used for each Vβ segment; one localized on the promoter region and one localized near the RSS. (B) The presence of trimethylated H3K27 on the four Vβ segments was analyzed by ChIP. Graphics present the fold enrichment of H3K27me3 on the eight different chromatin regions. Symbols correspond to three independent experiments. Median is indicated as a black line. (C,D) Relative expression of PRC2 components (EZH2, Suz12 and EED) studied by Western blotting and normalized to GAPDH. (C) Levels of EZH2, Suz12 and EED normalized to GAPDH. (D) Western blots representative of three independent experiments. Data are mean ± SD of three independent experiments. T-tests or Mann–Whitney tests were used to reveal statistically significant differences. * p < 0.05, ** p < 0.01.