Table 2.

Primers used to quantify TCRβ germline transcripts.

Target	Sequences	Length (bp)	Annealing Temperature (°C)
Vβ3	F 5′-ATGGATATCTGGCTTCTAGGT-3′ R 5′-GTTTGTGTACAGGAAGACGGT-3′	470	54
Vβ12-1	F 5′-CATCCTGAGAAGAAGCATGTC-3′ R 5′-TTACAGAAAGCCAGTAGCTTTG-3′	550	55
Vβ13-1	F 5′-CACTAAGTCACTGAAAGCCC-3′ R 5′-CCACACATCACTGTGCATC-3′	524	54
Vβ13-2	F 5′-TCCAGGCTCTTCTTCGTG-3′ R 5′-CCCCACATCACTGTGCATCA-3′	446	56
Vβ13-3	F 5′-AGCCCCAGTTCTAATTTACC-3′ R 5′-GAAGGAAGCCACACATCAC-3′	523	54
Vβ14	F 5′-GGCAGTGTTCTGTCTCCTTG-3′ R 5′-GAAAACCATCAGCTTTGTGC-3′	477	55
Vβ19	F 5′-AACAAGTGGGTTTTCTGCTG-3′ R 5′-ATGGAGAGGGGGTAGCTGT-3′	488	59
Vβ29	F 5′-GTTAGGCTCATCTCTGCTGTG-3′ R 5′-GAAAGGATGTGGCTGTGTAGA-3′	500	54
PPIA-2	F 5′-CACCGTGTTCTTCGACATCA-3′ R 5′-TTCTGTGAAAGGAGGAACCC-3′	155	62