Table 5.
Primers used for ChIP experiments.
| Target | Sequences | Length (bp) | Annealing Temperature (°C) | |
|---|---|---|---|---|
| Promoter | Vβ12-1 | F 5′-CACAGAAGGGCATAGCCAAC-3′ R 5′-TGCTGGTCCTCTTGGTGAGA-3′ |
179 | 61 |
| Vβ13-1 | F 5′-CACTAAGTCACTGAAAGCCC-3′ R 5′-CACTTGTACACAGGAGGCTC-3′ |
122 | 61 | |
| Vβ13-3 | F 5′-AGCCCCAGTTCTAATTTACC-3′ R 5′-CTGACCAGCACTCACTTGCA-3′ |
122 | 61 | |
| Vβ14 | F 5′-GACTGATTCTCTGAAGGGACA-3′ R 5′-CAAGGAGACAGAACACTGCC-3′ |
155 | 61 | |
| RSS region | Vβ12-1 | F 5′-CATGAGTGCCTTGGAACTGG-3′ R 5′-TTACAGAAAGCCAGTAGCTTTG-3′ |
87 | 62 |
| Vβ13-1 | F 5′-TCAGGAAGTCCCTGCCTCTA-3′ R 5′-TGGCCTTCGGGCAGCTAGAAA-3′ |
86 | 62 | |
| Vβ13-3 | F 5′-CTAGCTCTCTGTGTACCCCA-3′ R 5′-AGGATGAGACTCATGCTGTG-3′ |
91 | 60 | |
| Vβ14 | F 5′-GGCCTAAAGGAACTAACTCCA-3′ R 5′-ACCATCAGCTTTGTGCACAG-3′ |
130 | 62 | |