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. 2022 Jul 4;23(13):7433. doi: 10.3390/ijms23137433

Figure 1.

Figure 1

Schematic representation of the donor plasmids. (A) pBacMCS[Ser1-H1/P1A269/FLAG, 3 x P3-EGFP] and (B) pBacMCS[Ser3-H1/P1A269/FLAG, 3 x P3-DsRed] containing designed sequences expressing the pierisin-1A (P1A) catalytic domain under the control of the Ser1 and Ser3 promoters, respectively, along with the genetic markers. The numbers indicate the nucleotide position of the gene promoter sequence [29,30] and 2–269 amino acid positions of P1A [20]. Black arrows indicate the piggyBac inverted terminal repeat (ITR) at the left and right arms; broken arrows indicate the direction of the translation.