Figure 2.

Histone H2Bub1 levels increase strongly in the gene bodies of both Atxn7l3^−/− mESCs and Atxn7l3^−/− MEFs. (a,b) Depletion of the SAGA DUB subunit, ATXN7L3, results in retention of H2Bub1 by around three to five-fold in mESCs, and around eight to ten-fold in MEFs compared to the corresponding wild type (WT) cells. Representative IGV genomic snapshots of H2Bub1-binding profiles are shown at two selected genes (Fam8a1 and Zfp330), re-analyzed from GSE153584 [34]. Direction of the transcription is indicated by arrows. Group scaled tag densities on each gene either in mESCs, or in MEFs, are indicated on the left.