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Specific mRNA transcript levels coding human Runt-related transcription factor 2 (RUNX2), Vitronectin (VNT), and Osteonectin (SPARC) were evaluated by quantitative RT-PCR in RNA isolated from osteoblast cells cultured on plastic supports (CP), MnGC, or MnGC-BMP for 24 h. Data are reported as mean ± standard error of 3 independent experiments, each performed in duplicate, and were calculated using the ΔΔCT method. ° indicates p < 0.05 vs. CP; * indicates p < 0.02.
