Figure 11.

Calcium salt deposition was assessed by alizarin red staining in h-osteoblast cells cultured for 7 days on plastic supports (CP), MnGC, or MnGC-BMP. (A) Alizarin red was recorded using spectrophotometry. O.D.—optical density. Data are reported as mean ± standard error of 3 independent experiments, each performed in duplicate. ° indicates p < 0.02 vs. CP; * indicates p < 0.02. (B) In a parallel set of experiments, stained samples were visualized using a Leica microscope. Representative images. Scale bar = 37.5 μm.