Table 2.
Tissue heterogeneity of several metabolic markers. Primary antibody panel/list of the primary antibodies used for immunohistochemistry and WesTM analyses.
| Primary Antibody | Abbreviation | Manufacturer | Catalogue Number | Dilutions | Target/Function/Marker | |
|---|---|---|---|---|---|---|
| Wes | IHC | |||||
| Acetyl-CoA Carboxylase | ACC | Cell Signaling | #3676 | - | 1:100 | lipid metabolism; acetyl CoA carboxylation |
| Acyl-CoA Synthetase Short-Chain Family Member 2 | ACSS2 | Cell Signaling | #3658 | - | 1:200 | acetate consumption |
| Alanine, Serine, Cysteine-Preferring Transporter 2 | ASCT2 | Bethyl | #A304-353A | - | 1:250 | glutamine transporter |
| β-actin | Merck-Sigma-Aldrich | #A2228 | 1:50 | - | internal/loading control | |
| β-F1-ATPase | ATPb | Abcam | #14730 | - | 1:100 | mitochondrial oxidative phosphorylation |
| Carnitine Palmitoyltransferase 1A | CPT1A | Abcam | #128568 | 1:50 | 1:500 | lipid metabolism; fatty acid beta-oxidation |
| Cytochrome c oxidase subunit 4 | COX4 | Cell Signaling | #4850 | 1:50 | - | terminal oxidation reaction in the electron transport chain |
| Fatty Acid Synthase | FASN | Cell Signaling | #3180 | 1:50 | 1:100 | lipid metabolism; fatty acid synthesis |
| Glucose Transporter 1 | GLUT1 | Abcam | #652 | - | 1:400 | glucose transporter |
| Glutaminase | GLS | Abcam | #156876 | - | 1:200 | glutaminolysis |
| Hexokinase 2 | HK2 | Cell Signaling | #2867 | - | 1:200 | glycolysis |
| Lactate Dehydrogenase A | LDHA | Cell Signaling | #3582 | - | 1:400 | glycolysis |
| Lactate Dehydrogenase B | LDHB | Abcam | #85319 | 1:50 | - | glycolysis |
| Phospho-The37/46-4EBP1 | p-4EBP1 | Cell Signaling | #2855 | 1:50 | - | mTOR complex activity |
| Phosphofructokinase | PFKP | Cell Signaling | #8164 | - | 1:100 | glycolysis |
| Phospho-Ser79-Acetyl-CoA Carboxylase | p-ACC | Cell Signaling | #11818 | - | 1:100 | lipid metabolism; acetyl CoA carboxylation |
| Phospho-Ser235/236-Ribosomal S6 | p-S6 | Cell Signalling | #4858 | 1:50 | 1:100 | mTOR complex activity |
| Phospho-Ser473-Akt | p-Ser473-Akt | Cell Signaling | #4060 | 1:50 | - | mTOR complex activity |
| phospho-Ser2448-mTOR | p-mTOR | Cell Signaling | #2976 | 1:50 | 1:100 | mTOR complex activity |
| Pyruvate Kinase Isoenzyme M2 | PKM2 | Cell Signaling | #4053 | 1:50 | - | glycolysis |
| Rictor | Bethyl | #A300-458A | - | 1:1000 | mTOR complex activity | |