iCC	Intrahepatic cholangiocarcinoma
NO	nitric oxide
RBE	human intrahepatic cholangiocarcinoma cell lines
CCA	Cholangiocarcinoma
RFA	percutaneous radiofrequency ablation
NCCN	National Comprehensive Cancer Network
CSCO	Chinese Society of Clinical Oncology
GP	gemcitabine combining cisplatin
GS	gemcitabine combining diageo
ICIs	immune checkpoint inhibitors
PD-1	the programmed cell death 1
CTLA-4	the cytotoxic T-lymphocyte associated antigen 4
DIAD	diisopropyl azodicarboxylate
Ph3P	triphenylphosphine
HATU	2-(7-azabenzotriazol-1-yl)-N, N, N′, N′-tetramethyluronium hexafluorophosphate
DIPEA	N, N-diisopropylethylamine
PTX	paclitaxel
DOX	doxorubicin
MFI	Mean fluorescence intensity
MF	Metabolic bioavailability