. 2022 Jun 28;27(13):4148. doi: 10.3390/molecules27134148

Table 4.

FAME analysis of the acyl lipids extracted from lentisk leaves. The levels of each compound are expressed as chromatographic relative peak surface measured as total ion current (area % TIC). The extraction yield for each lipid fraction was similar: 1.95 ± 0.05 g/100 g of dry matter.

		Mountain (Site M)				Littoral (Site L)				Altitude * Season Interaction
Fatty Acid	Corresponding acyl	Winter	Spring	Summer	Autumn	Winter	Spring	Summer	Autumn
Methyl Ester (ME)	Corresponding acyl	Winter	Spring	Summer	Autumn	Winter	Spring	Summer	Autumn
Myristic acid ME	C14:0	6.6 ± 1.7 ^ac	4.0 ± 0.2 ^bc	1.8 ± 0.1 ^ac	3.4 ± 0.1 ^ab	2.5 ± 0.1 ^bc	3.2 ± 0.1 ^c	2.7 ± 0.3 ^a	2.7 ± 0.5 ^d	***
Hexadecenoic acid ME	C16:1	1.2 ± 0.3 ^c	0.9 ± 0.1 ^b	1.2 ± 0.1 ^bc	1.1 ± 0.1 ^a	0.5 ± 0.1 ^b	1.1 ± 0.1 ^ab	1.3 ± 0.1 ^bc	1.5 ± 0.2 ^bc	***
Palmitic acid ME	C16:0	24.8 ± 2.3 ^c	28.3 ± 0.3 ^c	27.4 ± 0.5 ^c	30.3 ± 0.9 ^a	26.3 ± 1.1 ^bc	31.5 ± 0.4 ^ac	31.4 ± 1.0 ^ab	31.7 ± 2.0 ^a
Margaric acid ME	C17:0	0.7 ± 0.1 ^c	0.8 ± 0.1 ^b	0.8 ± 0.1 ^bc	0.9 ± 0.1 ^a	0.6 ± 0.1 ^b	0.9 ± 0.1 ^ab	0.9 ± 0.1 ^ab	1.1 ± 0.1 ^ab	**
Linoleic acid ME	C18:2	20.1 ± 1.7 ^ab	20.0 ± 0.2 ^c	28.2 ± 8.7 ^a	13.9 ± 0.6 ^c	26.2 ± 0.9 ^ab	22.7 ± 0.5 ^bc	9.7 ± 0.2 ^c	14.1 ± 1.2 ^bc	***
Oleic acid ME	C18:1	2.8 ± 0.2 ^c	5.7 ± 0.4 ^bc	15.3 ± 8.3 ^d	10.8 ± 0.3 ^c	14.4 ± 0.3 ^bc	10.4 ± 0.6 ^ab	30.2 ± 1.2 ^c	15.3 ± 1.5 ^a	**
Linolenic acid ME	C18:3	35.5 ± 1.8 ^d	34.0 ±1.2 ^bc	18.5 ± 0.2 ^a	31.8 ± 1.1 ^c	23.4 ± 0.2 ^e	21.7 ± 0.6 ^e	17.6 ± 0.7 ^ab	27.2 ± 2.8 ^e	***
Stearic acid ME	C18:0	1.9 ± 0.2 ^bc	2.3 ± 0.1 ^ab	2.6 ± 0.1 ^c	2.6 ± 0.1 ^a	2.1 ± 0.1 ^bc	2.2 ± 0.2 ^a^b	2.8 ± 0.1 ^bc	2.6 ± 0.3 ^a
Docosanoic acid ME	C22:0	0.9 ± 0.1 ^ac	1.2 ± 0.1 ^c	1.1 ± 0.1 ^bc	1.2 ± 0.1 ^a	0.9 ± 0.1 ^bc	1.3 ± 0.1 ^c	1.2 ± 0.2 ^ac	1.2 ± 0.2 ^ab
	Total identified	94.3 ± 3.0	96.7 ± 0.7	96.8 ± 0.7	95.9 ± 0.6	96.9 ± 0.3	94.8 ± 0.9	97.8 ± 0.2	97.3 ± 0.3
C14; C16; C17; C18; C22	Mean (ΣSFA)	34.8 ± 1.0 ^d	36.6 ± 0.4 ^d	33.6 ± 0.7 ^d	38.3 ± 1.1 ^a	32.4 ± 1.2 ^cd	39.0 ± 0.7 ^bd	39.0 ± 1.5 ^ab	39.6 ± 2.9 ^abc	**
C16:1; C18:1	Mean (ΣMUFA)	3.9 ± 0.5 ^c	6.6 ± 0.4 ^ac	16.5 ± 8.3 ^d	11.9 ± 0.4 ^bc	14.9 ± 0.2 ^ac	11.5 ± 0.5 ^ab	31.5 ± 1.2 ^c	16.8 ± 1.7 ^a	*
C18:2; C18:3	Mean (ΣPUFA)	55.5 ± 2.7 ^b	54.0 ±1.1 ^bc	46.7 ± 8.6 ^a	45.7± 0.5 ^bd	49.6 ± 0.9 ^bd	44.3 ± 1.1 ^cd	27.3 ± 0.5 ^bd	41.2 ± 1.8 ^d	**

Means followed by different letters (in italic) within the same row were significantly different (p < 0.05). Significance effect of the interaction between altitude and season as determined by ANOVA, p-values: *** p < 0.001; ** p < 0.01; * p < 0.05.