TABLE 1.

PGA synthesis and GGT activity in cultures of some strains of B. licheniformis and B. subtilis^a

Strainb	PGA (μg/ml) in mediumc:				GGT (U/ml) in mediumc:
	1	2	3	4	1	2	3	4
B. licheniformis
DSMZ 13T	0	0	450	0	0	0	2.6	0.9
NCIMB 9375T	540	0	620	0	1.7	6.4	30.0	5.6
ATCC 9945a (A)	0	0	260	0	0	0	4.9	7.9
ATCC 9945a (B)	510	0	420	60	3.8	0	3.8	3.4
NCIMB 6346	370	0	790	0	2.2	2.8	3.2	5.4
NCTC 8223	530	0	420	0	2.5	0	3.5	1.5
S170	0	0	410	170	0	2.6	6.5	3.2
S173	260	0	390	90	3.6	17.3	3.9	4.4
S178	0	0	1,470	0	0	0	5.2	0.7
B. subtilis
NCIMB 3610T	0	360	300	300	0	1.1	1.0	3.0
NRS 264	0	1,900	0	0	0	1.7	3.2	1.1
S031	0	120	0	0	0	0.3	0	0
S317	0	0	390	280	0	0	0	0
NRRL B-1490	0	0	0	0	0	0	3.0	0.2
1E2 (pLS11)	0	0	20	0	0	0	2.4	1.6
1E3 (pLS12)	0	340	220	340	0	0.6	6.2	11.3

^aPGA and GGT activities are normalized to an OD₆₆₀ of 1.0. The limit of PGA detection was 20 μg/ml and that of GGT activity was 0.3 U/ml. 

^bStrains were from our culture collection and have been described previously (13) with the exception of strains 1E2 and 1E3 from the Bacillus Genetic Stock Center (Ohio State University). DSMZ 13^T and NCIMB 9375^T are independent cultures of the type strain and two strains of ATCC 9945a were used (see text for details). 

^cCultures were grown in basal salts medium containing the following (per liter): medium 1, (NH4)₂SO₄ (4 g); medium 2, sodium glutamate (4 g); medium 3, casein hydrolysate (4 g); medium 4, casein hydrolysate (4 g) plus sodium glutamate (4 g) for B. licheniformis strains or (NH4)₂SO₄ (4 g) for B. subtilis strains.