Figure 1.

Effects of PAG, AOAA, and L-Asp on viability, H₂S content, proliferation, and colony formation in human BC cells. (a) The CC-K8 assay was used to determine the percentage of viable cells. The cell viability of each group without PAG, AOAA, or L-Asp treatment was normalized as 100% and considered to be the control group. (b) The levels of H₂S in BC cells were detected. (c) The proliferation rate of each group was analyzed. (d) DNA replication activities of BC cells in each group were examined by EdU assay (original magnification ×200). (e) The clonogenic capacity was determined in BC cells. (f) The number of colonies was calculated. The experiments were performed in triplicate. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 compared with the control group; ^△△ p < 0.01 compared with PAG group; ^★★ p < 0.01 compared with AOAA group; ^## p < 0.01 compared with L-Asp group.