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. 2022 Jun 23;27(13):4049. doi: 10.3390/molecules27134049

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of PAG, AOAA, and L-Asp on the cell death of human BC cells (a) The apoptotic level was measured by TUNEL staining (original magnification ×200). (b) Apoptotic index was calculated. (c) The expression levels of Bax, Bcl-2, Bad, Bcl-xl, cleaved caspase-3, and cleaved PARP were detected by Western blot. β-actin was used as a loading control. (d) Densitometric quantification was performed, normalized to the level of β-actin. The experiments were performed in triplicate. Data are presented as mean ± SEM. ** p < 0.01 compared with the control group; ^△△ p < 0.01 compared with PAG group; ^★★ p < 0.01 compared with AOAA group; ^## p < 0.01 compared with L-Asp group.