Figure 6.

The ability of AgNPs prepared using extracts from L. indica (LI), A. scholaris (AS), and A. multifolium (AM) to disperse 48 h biofilms of S. aureus (A) and E. coli (B). Biofilm formation from all AgNPs analyzes in SA and EC (C). Absolute values for S. aureus OD₆₀₀ = 0.40 ± 0.05 for E. coli OD₆₀₀ = 0.70 ± 0.05. The effect was calculated relative to the negative control sample without nanoparticles (0%); calculations also considered the intrinsic density of nanoparticles adsorbed on the surface of the plate wells. LB medium, incubation at 37 °C. The staining was performed with crystal violet. All measurements were performed in no less than five replicates. For further details, see the section of Material and Methods.