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. 2022 Jun 25;12(13):2183. doi: 10.3390/nano12132183

Figure 7.

Figure 7

Spectrophotometric study of biofilm formation—Pyrogallol red method. Comparison of protein content in biofilm in 24 and 48 h against bacterial medium for E. coli (A). There are significant differences between protein content in media and biofilms and between 24 h and 48 h-biofilm. (B) Dependence of biofilm formation on CFU after 48 h. The protein content is increasing with a growing number of CFU. (C) Forest plot comparing the effect of using 20 μg/mL of AgNPs from L. indica (LI), A. scholaris (AS), and A. multifolium (AM) on biofilms of S. aureus (SA) and E. coli (EC) against non-treated biofilms in 24 h and (D) 48 h. (E) Boxplots comparing the overall area of non-treated biofilms and biofilms treated with 20 μg/mL AgNPs from LI, AS, and AM during 24 h and (F) 48 h.