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. 2022 Jun 21;27(13):3988. doi: 10.3390/molecules27133988

Figure 4.

Figure 4

Apoptosis evaluation in SK-Hep-1 (HTB-52) (10,000 events) after treatment with L-R and L-DOX. After 24 h of treatment, the cells were stained with Annexin V-FITC and Ethidium Homodimer III (EthD-III) and were evaluated using a BD FACS Canto II flow cytometer (Becton Dickinson, USA) using the BD FACSDiva 6.1.2 software. The results are shown in dot plots divided into 4 quadrants: Q3—viable cells, (Annexin V-FITC (−), EthD-III (−)), Q1—early apoptotic cells (Annexin V-FITC (+), EthD-III (−)), Q2—late apoptotic (Annexin V-FITC (+), EthD-III (+)), and Q4—necrotic cells (Annexin V-FITC (−), EthD-III (+)).