Table 4.

Acute toxicity of methanolic extract and n-butanol fractions of Chrozophora tinctoria in pigeons.

Sample	Dose (g)·(mL)/kg	Emesis	Diarrhea	Lethargy	Mortality (%)
		Total Number of Vomits	Total Number of Wet Stools
Distal Water	6	0.00 ± 0.00	0.00 ± 0.00	-	0.00 ± 0.00
MEC	0.3	0.00 ± 0.00	0.00 ± 0.00	-	0.00 ± 0.00
	0.5	0.00 ± 0.00	0.00 ± 0.00	-	0.00 ± 0.00
	1	4.00 ± 1.00	9.33 ± 2.52 *	-	0.00 ± 0.00
	2	5.00 ± 2.00	13.33 ± 2.52 ***	-	0.00 ± 0.00
	3	7.00 ± 2.00 **	14.00 ± 4.58 ***	Less	0.00 ± 0.00
	4	7.67 ± 2.52 **	14.33 ± 4.51 ***	More	50.00 ± 0.00
	5	9.00 ± 2.65 ***	16.33 ± 2.52 ***	More	50.00 ± 0.00
NBFC	0.3	0.00 ± 0.00	0.00 ± 0.00	-	0.00 ± 0.00
	0.5	0.00 ± 0.00	0.00 ± 0.00	-	0.00 ± 0.00
	1	3.00 ± 1.00	10.33 ± 3.51 *	-	0.00 ± 0.00
	2	4.67 ± 1.53	14.67 ± 3.06 ***	Less	0.00 ± 0.00
	3	5.00 ± 1.73	15.00 ± 5.00 ***	More	25.00 ± 0.00
	4	6.00 ± 2.00 *	16.00 ± 3.61 ***	Most	50.00 ± 0.00
	5	7.33 ± 3.51 **	19.33 ± 3.51 ***	Most	75.00 ± 0.00

Values are expressed as mean ± SEM. Statistical significance was determined using GraphPad Prism 6.01 software (one-way ANOVA followed by Tukey’s multiple comparison test), by Dotmatics, San Diego, CA, USA. * p ≤ 0.05 was considered statistically significant. (** p ≤ 0.01, *** p ≤ 0.001). MEC = methanolic extract of C. tinctoria; AChE = Acetylcholinesterase; NBFC = n-butanol fraction of Chrozophora tinctoria.