Table 8.
Diarrheal/laxative activity of n-butanol fraction of C. tinctoria in loperamide hydrochloride in-duced constipation in pigeons.
| Samples | Dose g/kg mL/kg (P/O) |
First Stool/Latency Time (Minutes) |
Total Number of Stools | Number of Wet Stools | Weight of Stools (Grams) |
Percent of Wet Stool (%) |
|---|---|---|---|---|---|---|
| NBFC + DW | 1 | 28.33 ± 2.52 | 17.00 ± 3.00 | 10.33 ± 1.53 | 13.37 ± 1.96 | 61.03 ± 2.87 |
| 2 | 26.33 ± 3.51 | 19.00 ± 2.00 | 14.33 ± 1.53 | 16.13 ± 2.70 | 75.45 ± 1.54 | |
| 3 | 23.33 ± 2.52 | 20.00 ± 1.00 | 16.33 ± 1.53 | 17.17 ± 2.16 | 81.55 ± 3.64 | |
| Loperamide hydrochloride (4 mg/kg) was administered (P.0) 30 min before fractions/distilled water/castor oil | ||||||
| Distilled Water (-ive Control) | 6 | 70.33 ± 2.52 | 8.33 ± 2.52 | 00 ± 00 | 7.50 ± 2.29 | 00 ± 00 |
| Castor oil (+ive Control) | 6 | 17.33 ± 2.52 *** | 16.00 ± 2.00 ** | 14.33 ± 1.53 *** | 15.07 ± 1.27 *** | 89.74 ± 2.78 *** |
| NBFC + Lopr | 1 | 32.33 ± 2.52 *** | 12.67 ± 2.08 | 5.67 ± 1.53 ** | 9.50 ± 1.65 | 44.34 ± 7.11 *** |
| 2 | 30.67 ± 2.08 *** | 16.33 ± 2.52 *** | 8.33 ± 1.53 *** | 11.33 ± 1.15 * | 50.88 ± 1.52 *** | |
| 3 | 24.33 ± 2.52 *** | 15.00 ± 2.00 ** | 13.33 ± 1.53 *** | 14.00 ± 2.17 *** | 89.06 ± 2.91 *** | |
Data are represented as mean ± SEM. The data were analyzed by one-way ANOVA followed by Dunnett’s test using GraphPad Prism version 6.01; p ≤ 0.05 was considered significant (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001). NBFC, n-butanol fraction of Chrozophora tinctoria; Lopr, loperamide hydrochloride; DW, distilled water; PO, per orally/by orally.