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. 2022 Jun 30;27(13):4205. doi: 10.3390/molecules27134205

Figure 4.

Figure 4

Effect of agerarin and AHE on the suppression of IL-4-induced PER2 expression. (A,C) HaCaT cells were pretreated with 20 or 40 μg/mL AHE (A) and 20 or 40 μM agerarin (C) for 30 min before stimulation in the presence (+) or absence (−) of 20 ng/mL IL-4. After 3 h, total RNA was isolated, and the levels of PER2 mRNA were measured by RT-PCR. The mRNA level of GAPDH was used as an internal control. (B,D) HaCaT cells were pretreated with AHE (B) and agerarin (D), as described above. After 24 h, whole-cell lysates were prepared and immunoblotted using anti-PER2 antibodies. GAPDH was used as the loading control. Band intensities of PER2 protein were measured using ImageJ and normalized to GAPDH. ns, not significant; *** p < 0.001 compared to IL-4-treated group (n = 3) by Sidak’s multiple comparisons test.