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. 2022 Jun 23;27(13):4054. doi: 10.3390/molecules27134054

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of coumarin derivatives on the expression of glycoproteins on human platelet membranes. Human blood was treated with 10 ppm of coumarin derivatives for 30 min, followed by the addition of 20 μM ADP for 30 min. The expression of GPIIIa and active form of GPIIb/IIIa on the membrane was analyzed using flow cytometry. The flow data for GPIIIa and GPIIb/IIIa on platelet membranes from one of three independent experiments (n = 3) (A). Experimental results are summarized as MFI values of platelet membrane (B). * p < 0.05 compared with the control group. # p < 0.05 compared with whole blood stimulated with ADP alone.