Figure 1.

Increased extracellular [Na⁺] increased [Ca²⁺]_i in single AgRP and POMC neurons isolated from ARC of wild-type mice.(A,B) Left panel: Typical examples of [Ca²⁺]_i responses to [Na⁺] increase and 10⁻⁵ M glutamate (GA) in single ARC neurons. Right panel: The single ARC neurons were subsequently shown to be IR to AgRP (A) and POMC (B). Scale bar is 20 μm. (C) Average amplitude of [Ca²⁺]_i responses to increased extracellular [Na⁺] (Δratio) in AgRP and POMC neurons. Data are presented as mean ± SEM.