Figure 2.

Increased extracellular [Na⁺] failed to substantially increase [Ca²⁺]_i in single POMC neurons in ARC from TRPV1 KO mice. (A,B) Typical examples of [Ca²⁺]_i responses to [Na⁺] increase and 10⁻⁵ M glutamate (GA) in single ARC POMC neuron from wild-type mice (A) and TRPV1 KO mice (B). Scale bar is 20 μm. (C) Average amplitude of [Ca²⁺]_i responses to increased extracellular [Na⁺] (Δratio) in POMC neurons. Data are presented as mean ± SEM. WT vs. KO determined by one-way ANOVA followed by the Bonferroni test.