Skip to main content
. 2022 Jun 24;15:898851. doi: 10.3389/fnmol.2022.898851

FIGURE 4.

FIGURE 4

Chronic social stress alters Ahnak expression in the hippocampus. (A) Diagram and timeline of CSDS and tissue collection. (B–D) Immunoblotting of Ahnak protein using hippocampal lysates. (B) Representative images of immunoblotting of Ahnak and Gapdh as a control. (C) Quantification of immunoblots [one-way ANOVA, Non-defeated (n = 23 mice), Res (n = 23 mice) and Sus (n = 15 mice)]. (D) Hippocampal Ahnak is inversely correlated with SI ratio (pearson r: r = –0.4172, p = 0.0008, n = 61 mice) (E) Representative images PV and Ahnak mRNA expression using RNAScope. Scale bar, 5 μm. (F) Ahnak mRNA expression in vDG PV neurons from Non-defeat, Res and Sus groups (Kruskal–Wallis test with post hoc Dunn’s multiple comparisons: Non-defeated (n = 102 cells), Res (n = 94 cells) and Sus (n = 73 cells). (G) Ahnak mRNA expression in vDG PV neurons is inversely correlated with SI ratio (pearson r: r = –0.8401, p = 0.0240, n = 10 mice). All data were normalized to ND group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.