. 2022 Jun 23;11(13):1667. doi: 10.3390/plants11131667

Table 2.

Bioactive compounds of methanolic leaf extract of Skimmia anquetlia identified using GC-MS analysis.

S. N.	Retention Time (min)	Phytocompounds	CAS Number	Peak Area (%)	RSI	Molecular Formula	Molecular Weight (g/mol)
1.	3.490	1,3,5-Cycloheptatriene	544-25-2	3.76	914	C₇H₈	92.14
2.	5.150	2-Propenoic acid, butyl ester	141-32-2	0.86	890	C₇H₁₂O₂	128.16
3.	8.453	Geijerene	6902-73-4	0.62	-	C₁₂H₁₈	162.2713
4.	10.595	Linalyl acetate	115-95-7	1.85	937	C₁₂H₂₀O₂	196.29
5.	10.950	Linalool	78-70-6	1.65	858	C₁₀H₁₈O	154.25
6.	11.813	Glycerol 1,2-diacetate	102-62-5	0.79	941	C₇H₁₂O₅	176.16
7.	12.152	Geranyl acetate	105-87-3	0.44	925	C₁₂H₂₀O₂	196.29
8.	12.891	Methyl (2E,5E)-2,5-octadecadienoate	56846-97-0	0.64	-	C₁₉H₃₀O₂	294.5
9.	14.636	3,7,11-Trimethyl-3-hydroxy-6,10-dodecadien-1-yl acetate	0	0.57	939	C₁₇H₃₀O₃	282.41
10.	15.493	3-Hydroxypropanoic acid 1-butyl ester	0	0.76	819	C₇H₁₄O₃	146.18
11.	18.489	Hexadecanoic acid, methyl ester	112-39-0	0.64	916	C₁₇H₃₄O₂	270.45
12.	19.255	2H-1-Benzopyran-2-one, 5,7-dimethoxy-	487-06-9	0.61	984	C₁₁H₁₀O₄	206.19
13.	20.051	7H-Furo [3,2-g][1]benzopyran-7-one, 4-methoxy-	484-20-8	2.79	942	C₁₂H₈O₄	216.18
14.	20.204	5,10-Pentadecadienal, (Z,Z)-	64275-49-6	1.69	911	C₁₅H₂₆O	222.37
15.	20.312	Photocitral A	55253-28-6	2.55	948	C₁₀H₁₆O	152.23
16.	21.391	2H-1-Benzopyran-2-one, 7-methoxy-6-(3-methyl-2-butenyl)-	581-31-7	1.32	923	C₁₅H₁₆O₃	244.28
17.	21.439	Tetradecanoic acid	544-63-8	0.33	-	C₁₄H₂₈O₂	228
18.	21.559	2R-Acetoxymethyl-1,3,3-trimethyl-4t-(3-methyl-2-buten-1-yl)-1t-cyclohexanol	0	23.9	932	C₁₇H₃₀O₃	282.4
19.	21.799	1,3,3-Trimethyl-2-hydroxymethyl-3,3-dimethyl-4-(3-methylbut-2-enyl)-cyclohexene	0	1.59	946	C₁₅H₂₆O	222.37
20.	22.027	Pentanedioic acid, 2,2-dimethyl-, dimethyl ester	13051-32-6	0.93	824	C₈H₁₄O₄	174.19
21.	22.363	Isoauraptene	1088-17-1	1.77	889	C₁₅H₁₆O₄	260.28
22.	22.462	10-Pentadecen-5-yn-1-ol, (E)-	64275-59-8	0.68	880	C₁₅H₂₆O	222.37
23.	23.493	Nonacos-1-ene	18835-35-3	0.62	935	C₂₉H₅₂	400.72
24.	23.663	2,6,10,14-Tetramethylpentadecan-6-ol	104000-14-8	0.68	892	C₁₉H₄₀O	284.5
25.	24.078	1-Dodecanol, 3,7,11-trimethyl-	6750-34-1	0.89	868	C₁₅H₃₂O	228.41
26.	24.220	8-(2,3-Dihydroxy-3-methylbutyl)-7-methoxy-2H-chromen-2-one	5673-37-0	8.47	813	C₁₅H₁₈O₅	278.30
27.	25.122	10-Pentadecen-5-yn-1-ol, (E)-	64275-59-8	1.69	974	C₁₅H₂₆O	222.37
28.	25.254	Wampetin	89824-26-0	4.73	860	C₂₁H₁₈O₆	366.4
29.	26.060	Squalene	111-02-4	9.98	933	C₃₀H₅₀	410.72
30.	27.445	(3E,5E,7E)-6-Methyl-8-(2,6,6-trimethyl-1-cyclohexenyl)-3,5,7-octatrien-2-one	17974-57-1	0.90	858	C₁₈H₂₆O	258.399
31.	27.747	Cyclohexene, 1,5,5-trimethyl-6-(2-propenylidene)-	56248-17-0	7.79	885	C₁₂H₁₈	162.27
32.	28.608	Clionasterol acetate	4651-54-1	6.59	888	C₃₁H₅₂O₂	456.7
33.	30.057	Ergost-5-en-3-ol, (3ß)-	4651-51-8	0.85	890	C₂₈H₄₈O	400.7
34.	31.237	2-Isopropyl-5-methylcyclohexyl 3-(1-(4-chlorophenyl)-3-oxobutyl)-coumarin-4-yl carbonate	0	3.43	896	C₃₀H₃₃ClO₆	525
35.	31.366	6ß-Hydroxy-17-oxo-4,5-secoandrostan-4-oic acid	59251-83-1	2.53	821	C₁₉H₃₀O₄	322.4

Identification of compounds was carried out by GC-MS spectrum. In the GC-MS spectrum, the requisites should be that RSI values were more than 800 or 900 according to NIST library guidelines.