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. 2001 Mar;67(3):1025–1029. doi: 10.1128/AEM.67.3.1025-1029.2001

FIG. 4.

FIG. 4

Effect of reconstitution of the ALSIIm-14 protein by trans-splicing on the growth of E. coli ER2744. (A) E. coli ER2744 transformed with plasmids expressing ALSII (sector 1), ALSIIm (sector 2), ALSIIm(N)-INn and INc-ALSII(C) (sector 3), ALSIIm(N)-INn (sector 4), INc-ALSII(C) (sector 5), or ALSIIm(N) and ALSII(C) (sector 6) were plated on M9 agar medium and incubated at 37°C (plate a), at 37°C with 100 μg of valine per ml (plate b), at 30°C with 100 μg of valine per ml (plate c), at 25°C with 100 μg of valine per ml (plate d), and at 30°C with 100 μg of valine per ml and 50 μg of SM per ml (plate e). All plates contained 0.3 mM IPTG. (B) E. coli ER2744 transformed with expression plasmids for fusion proteins as indicated on the figure were cultured in M9 medium containing 0.3 mM IPTG supplemented with valine and SM as indicated at the bottom. The cell optical density at 600 nm (OD600) was measured after incubation for 40 h at 30°C.