Figure 3.

The cytotoxic effects of different S. purpurea extracts against 4T1 cells. (A) Trypan blue dye exclusion staining. The cytotoxic effects of different S. purpurea extracts against 4T1 cells were estimated with trypan blue assay after 24 h of incubation. The 4T1 cells incubated with S. purpurea-root-acetone of 100 mg/mL were almost dead. (B) The death rates of 4T1 cells. The anti-4T1 activity of S. purpurea extracts followed the order: roots > stems > leaves. The solvent used for extract preparations was also an important factor. As compared, the acetone fractions showed the highest anti-4T1 activities. The water extracts of S. purpurea did not cause any cytotoxic effects on the survival of 4T1 cells.