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. 2001 Mar;67(3):1052–1062. doi: 10.1128/AEM.67.3.1052-1062.2001

FIG. 3.

FIG. 3

PCR-DGGE analysis of the bacterial diversity in enrichment cultures of FSL soils treated with petroleum supplemented with DBT (duplicate day-15 microcosms used). Lanes: M, marker (products of, from top to bottom, Enterobacter cloacae BE1, Listeria innocua ALM105, Rhizobium leguminosarum bv. trifolii R62, Arthrobacter sp., Burkholderia cepacia P2); 1, FSL soil, untreated; 2, FSL soil, treated, time zero; 3, FSL soil, treated, 15 days (replicate 1); 4 to 7, enrichment culture from FSL, treated, 15 days (replicate 1), from first to fourth culture, respectively; 8, FSL, treated, 15 days (replicate 2); 9 to 12, enrichment culture from FSL, treated, 15 days (replicate 2), from first to fourth culture, respectively; 13, band 3 (sequence similarity to R. erythropolis); 14, band 7 (similar to P. aureofaciens); 15, band 2 (similar to P. putida); 16, band 6 (similar to P. rhodesiae); 17, band 5 (similar to S. maltophilia); 18, band 4 (similar to B. brennerae DSM9396); 19, product of strain M39; 20, product of strain M41; 21, product of strain A36; 22, product of strain A69; 23, product of strain A96.