TABLE 1.
Specific PCR primers for the detection of dszA, dszB, and dszC genes based on the sequence of the R. erythropolis IGTS8 dsz operona
| Gene | Primer orientation | Position | Product size (bp) | Sequence |
|---|---|---|---|---|
| dszA | Forward | 371–388 | 5′-TCGATCAGTTGTCAGGGG-3′ | |
| Reverse | 898–917 | 547 | 3′-GGATGGACCAGACTGTTGAG-5′ | |
| dszB | Forward | 139–158 | 5′-ATCGAACTCGACGTCCTCAG-3′ | |
| Reverse | 541–560 | 422 | 3′-GGAACATCGACACCAGGACT-5′ | |
| dszC | Forward | 277–296 | 5′-CTGTTCGGATACCACCTCAC-3′ | |
| Reverse | 651–668 | 392 | 3′-ACGTTGTGGAAGTCCGTG-5′ |
a
The forward primers used for DGGE analysis contained a GC clamp (27).