TABLE 5.
Strains isolated from enrichment cultures and their capacity to desulfurize DBT
| Strain | Enriched and isolated from: | Species bya:
|
SIb | dsz genes presentc | Desulfurization capacity (%)d | |
|---|---|---|---|---|---|---|
| Sequence | FAME | |||||
| A36 | A soil | R. erythropolis | R. erythropolis | 0.503 | dszABC | 63 |
| A61 | A soil | R. erythropolis | dszABC | 25 | ||
| A69 | A soil | R. erythropolis | R. erythropolis | 0.489 | dszABC | 78 |
| A74 | A soil | R. erythropolis | 0.584 | dszAB | 64 | |
| A96 | A soil | R. erythropolis | R. erythropolis | 0.489 | dszABC | 20 |
| M39 | FSL + DBT | R. erythropolis | R. erythropolis | 0.136 | dszABC | 53 |
| M41 | FSL + DBT | R. erythropolis | R. erythropolis | 0.136 | dszABC | 14 |
| A66 | A soil | NI∗ | dszAB | 33 | ||
| A73 | A soil | NI∗ | dszAB | ND | ||
| M16 | FSL + DBT | S. maltophilia | dszB | 4 | ||
| M26 | FSL + DBT | S. maltophilia | dszB | 3 | ||
a
Sequence, match on DGGE with products of R. erythropolis and S. maltophilia; FAME, closest relative from FAME database; NI, not identified.
b
SI, similarity index, the level of similarity to closest hit of FAME database.
c
As evidenced by PCR with dszA-, dszB-, and dszC-specific PCR systems.
d
The percentage of DBT transformed, under standard conditions, into products by the IGTS8 pathway. R. erythropolis IGTS8 used as the positive control, transformed >25% of DBT under these conditions. ND, not determined.