Table 1. Relative gene expression levels (2-ΔCt) are presented as mean ± SEM for each cell type.
Comparisons are between fetal fibroblasts (n = 6) with adult fibroblasts (n = 4), and then fetal GREL cells (n = 7) with adult ovarian surface epithelial cells (n = 5) or granulosa cells (n = 5).
| Gene | Fetal fibroblasts | Adult fibroblasts | Fetal GREL cells | Adult ovarian surface epithelial cells | Adult granulosa cells |
|---|---|---|---|---|---|
| KRT19 | 0.542±0.203 | 0.286±0.120 | 5.044±1.313 | 3.090±0.515 | 0.003±0.001** |
| DSG2 | 0.059±0.024 | 0.027±0.011 | 0.132±0.048 | 0.041±0.005 | 0.000±0.000* |
| PKP2 | 0.022±0.005 | 0.01±0.002 | 0.080±0.024 | 0.106±0.013 | 0.012±0.003*** |
| OCLN | 0.001±0.000 | 0.006±0.005 | 0.017±0.005 | 0.272±0.057*** | 0.003±0.000** |
| MUC1 | 0.004±0.001 | 0.005±0.003 | 0.009±0.002 | 0.180±0.027*** | 0.002±0.001*** |
| GJA1 | 2.640±0.332 | 3.732±0.798 | 1.461±0.319 | 1.507±0.287 | 17.049±5.306** |
| CTNNB1 | 0.281±0.047 | 0.177±0.023 | 0.290±0.064 | 0.504±0.071 | 0.922±0.302* |
| LAMB2 | 0.193±0.026 | 0.130±0.037 | 0.165±0.037 | 0.856±0.191** | 0.650±0.107* |
| FBN1 | 0.913±0.103 | 1.425±0.161* | 0.374±0.104 | 0.211±0.029 | 0.012±0.003** |
| COL1A1 | 16.111±2.289 | 14.009±2.382 | 4.653±1.984 | 3.907±0.640 | 0.043±0.021*** |
| COL3A1 | 6.116±1.553 | 4.484±0.453 | 3.613±1.339 | 2.186±0.381 | 0.009±0.003* |
| COL4A1 | 1.797±0.845 | 0.329±0.081 | 0.752±0.418 | 0.301±0.129 | 0.441±0.127 |
| NR5A1 | 0.061±0.009 | 0.028±0.006* | 0.016±0.007 | 0.120±0.021** | 0.416±0.105*** |
| STAR | 0.038±0.011 | 0.007±0.002 | 0.018±0.005 | 0.009±0.002 | 0.002±0.001*** |
| CYP11A1 | 0.005±0.002 | 0.039±0.017 | 0.001±0.001 | 0.127±0.077*** | 3.758±1.128*** |
| HSD3B1 | 0.000±0.000 | 0.000±0.000 | 0.000±0.000 | 0.005±0.002*** | 0.316±0.116*** |
| CYP19A1 | 0.001±0.000 | 0.000±0.000 | 0.000±0.000 | 0.056±0.051* | 10.307±2.106*** |
| HSD17B1 | 0.000±0.000 | 0.000±0.000 | 0.000±0.000 | 0.003±0.001*** | 0.328±0.157*** |
| INHBA | 0.002±0.000 | 0.001±0.000 | 0.001±0.000 | 0.008±0.005 | 0.005±0.002 |
| NR2F2 | 0.559±0.128 | 0.455±0.096 | 0.135±0.037 | 0.172±0.024 | 0.050±0.016 |
| FOXL2 | 0.051±0.008 | 0.024±0.006* | 0.012±0.006 | 0.028±0.006 | 1.015±0.280*** |
| ESR1 | 0.001±0.001 | 0.002±0.001 | 0.014±0.007 | 0.570±0.094** | 0.116±0.010* |
| ESR2 | 0.007±0.001 | 0.003±0.000** | 0.009±0.006 | 0.022±0.003* | 0.144±0.039*** |
| TGFBR1 | 0.148±0.033 | 0.06±0.008* | 0.153±0.036 | 0.136±0.013 | 0.088±0.015 |
| TGFBR2 | 0.105±0.026 | 0.05±0.011 | 0.129±0.037 | 0.533±0.076* | 0.007±0.006*** |
| TGFBR3 | 0.195±0.058 | 0.065±0.038 | 0.125±0.027 | 0.483±0.096** | 0.325±0.083* |
| CCND2 | 0.096±0.011 | 0.018±0.003** | 0.038±0.011 | 0.111±0.032 | 0.480±0.156** |
| CCNE1 | 0.038±0.005 | 0.009±0.002** | 0.018±0.004 | 0.025±0.011 | 0.024±0.006 |
| CCNE2 | 0.039±0.005 | 0.005±0.001** | 0.024±0.005 | 0.022±0.007 | 0.060±0.0170 |
| ALDH1A1 | 0.095±0.026 | 0.102±0.030 | 0.019±0.003 | 0.038±0.011 | 0.008±0.002 |
| LGR5 | 0.000±0.000 | 0.000±0.000 | 0.001±0.000 | 0.045±0.007* | 0.000±0.000 |
| OCT4 | 0.012±0.001 | 0.007±0.001* | 0.011±0.003 | 0.039±0.006** | 0.014±0.003 |
| DAZL | 0.003±0.001 | 0.001±0.000 | 0.001±0.000 | 0.003±0.001 | 0.001±0.000 |
| VASA | 0.001±0.000 | 0.000±0.000* | 0.001±0.000 | 0.001±0.001 | 0.000±0.000 |
Student’s t-tests were used to analyse the differences between fetal fibroblasts and adult fibroblasts. One-way ANOVA with Tukey’s post-hoc test was used to analyse the differences between GREL cells with adult ovarian surface epithelial cells or granulosa cells
* P < 0.05
** P < 0.01
*** P < 0.001 report significant differences between the corresponding fetal cells with the adult cells.