TABLE 1.
Strains, vectors, and their characteristics
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli TG1 | supE hsdΔ5thi Δ(lac-proAB)F′ [traD36 proAB+lacIqlacZΔM15] | 21a |
| B. pumilus ATCC 15884 | Wild type, gram positive | American Type Culture Collection |
| Plasmids | ||
| PJDC9 | Emr, ΔlacZ | 11 |
| pHT315 | Emr, ΔlacZ | 2 |
| PJPDC1 | pJDC9 containing the 2.3-kp Sau3AI fragment of L. plantarum CHL2 DNA containing the pdc gene | 8 |
| pJPAD14 | pJDC9 containing the 1,230-bp PstI-HindIII fragment of pJPDC1 with the pdc gene | This study |
| PHPADBS | pHT315 containing the 5-kb Sau3AI fragment of B. subtilis DNA with the pad gene | 10 |
| PJPADBP | pJDC9 containing the 925-bp fragment of B. pumilus with the pad gene | This study |
| PJPADP1 | PJDC9 containing the 6-kb HindIII fragment of P. pentosaceus with the padA gene | 5 |
| pJPADP6 | pJDC9 containing the 970-bp fragment of P. pentosaceus with the pad gene | 5 |
| pJLP113PP | pJDC9 containing pdc promoter and the DNA fragment from L. plantarum coding for the first 113 amino acids of LPPAD followed by the DNA fragment from P. pentosaceus coding for the last 65 amino acids of PPPAD | This study |
| pJPP113LP | pJDC9 containing padA promoter and the DNA fragment from P. pentosaceus coding for the first 113 amino acids of PPPAD followed by the DNA fragment from L. plantarum coding for the last 61 amino acids of LPPAD | This study |
| pJBP121LP | pJDC9 containing pad promoter and the DNA fragment from B. pumilus coding for the first 121 amino acids of BPPAD followed by the DNA fragment from L. plantarum coding for the last 46 amino acids of LPPAD | This study |
| pJBS121LP | pJDC9 containing pad promoter and the DNA fragment from B. subtilis coding for the first 121 amino acids of BSPAD followed by the DNA fragment from L. plantarum coding for the last 46 amino acids of LPPAD | This study |