Table 2. Primer sequences used for quantitative real time RT-PCR.
Name | Forward Primer (5’-3’) | Reverse Primer (5’-3’) |
---|---|---|
FAS | ACCTCATCACTAGAAGCCACCAG | GTGGTACTTGGCCTTGGGTTTA |
ACC | TACAACGCAGGCATCAGAAG | TGTGCTGCAGGAAGATTGAC |
CPT1a | CTGCCAGTTCCATTAAGCCACA | CAGCTATGCAGCCTTTGACTACCA |
PPARα | GGCAATGCACTGAACATCGAG | GCCGAATAGTTCGCCGAAAG |
PPARδ | TGGCCCTGTTCCTAGAATTGATG | GCAAACTCTGCCTGTGAGCTG |
Dio2 | CTGTGGTTGGATGTAGTCACACGA | CTTTGCACCAGGACCCAAATG |
β-actin | ACGGTCAGGTCATCACTATCG | GGCATAGAGGTCTTTACGGATG |
Nox4 | ACTGGTGAAGATTTGCCTGGAAG | CACAGTATAGGCACAAAGGTCCAGA |
Agtr1a | CCCACTCAAGCCTGTCTACGAA | GTGTGCTTTGAACCTGTCACTCC |
Agtr1b | TCACACGCAGGCTTGTCAAC | CACTTCACTTGCAGGCTTTGAAC |
UCP1 | TGTGCAATGACCATGTACACCAA | GCACACAAACATGATGACGTTCC |
PGC-1α | GCACTGACAGATGGAGACGTGA | TCATTGTAGCTGAGCTGAGTGTTGG |
CD36 | AACCCAATGGAGCCATCTTTGA | GTTGAGCACACCTTGAACAAATGAG |
ACOX1 | GGCCGCTATGATGGAAATGTG | GGGCTTCAAGTGCTTGTGGTAA |