Table 2. Methods of platelet-rich plasma preparation, and anticoagulants and activating agents used in the previous studies.

Studies	Platelet-rich plasma preparation method	Anticoagulant used	Activating agent
Rha et al. (2013) [16]	Single spin	Citrate dextrose	Calcium chloride
Kesikburun et al. (2013) [14]	Single spin	Citrate dextrose	None
Scarpone et al. (2013) [13]	Single spin	Citrate dextrose	None
Ilhanli et al. (2015) [15]	Single spin	Citrate dextrose	Calcium chloride
Wesner et al. (2016) [18]	Single spin	Citrate dextrose	None
Shams et al. (2016) [17]	Single spin	Citrate dextrose	None
Say et al. (2014) [12]	Single spin	Citrate dextrose	Calcium chloride
Nejati et al. (2017) [11]	Double spin	Citrate dextrose	None
Current study	Double spin	Citrate dextrose	Calcium gluconate