FIG. 3.

Restriction digestion analysis and Southern hybridization. (A) Analysis on a 0.7% agarose gel of EcoRI-PstI-digested plasmids. (B and C) Hybridization with tdnQ (B) and IS1071 (C). Lane 1, C. testosteroni I2 (plasmid pNB2); lane 2, D. acidovorans LME1 (plasmid pNB1); lane 3, D. acidovorans B8c (plasmid pB8c); lane 4, D. acidovorans CA28 (plasmid pC1); lane 5, D. acidovorans BN3.1 (plasmid pB1); lane a, DIG-labeled Marker II (Hoffmann-La Roche); lane b, 1-kb extended marker. The Southern blot in panel C was obtained from a gel different from that shown in panel A but containing the same DNA samples.