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. 2022 Jun 14;298(7):102139. doi: 10.1016/j.jbc.2022.102139

Figure 4.

Figure 4

ES-Cu–mediated increase in intracellular Fe content is dependent on the high affinity Fe import machinery. BY4741 WT, ctr1Δ, and ftr1Δ cells were grown in YPD ± 0.25 μM ES-Cu for 10 h before measuring Cu (A) and Fe (B) by ICP-MS. BY4741 WT and fet4Δ cells were grown in YPD ± 0.25 μM ES-Cu for 10 h before measuring Cu (C) and Fe (D) levels by ICP-MS. Data are expressed as mean ± SD; (n = 3), ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, ns = not significant. ES-Cu, elesclomol-copper complex; ICP-MS, inductively coupled plasma-mass spectrometry.