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. 2022 Jul 8;13(7):591. doi: 10.1038/s41419-022-05044-9

Fig. 1. Differential response of glioma cells to TMZ treatment.

Fig. 1

A Representative images of colonies formed in each group treated with 5 and 20 µM TMZ. B Quantification of total colonies after TMZ treatment (5, 10, and 20 µM) in comparison to controls. C Histograms represent cell cycle distribution with TMZ treatment for 72 h. D Cell cycle distribution was conducted by flow cytometer analysis after TMZ treatment (100 μM) for 72 h. E Flow cytometry analysis of the percentage of γH2AX positive cells upon treatment with TMZ (100 μM) for 48 h. F Cell viability was analyzed 120 h after treatment with TMZ (200 μM) and measured by XTT assay. Values are mean ± SEM of three independent experiments, ns = not statistically significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Each dot represents an independent experiment.