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. 2022 Jul 8;13:3975. doi: 10.1038/s41467-022-31701-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Average number of unique crosslinks after acquisition using a stepped HCD MS2 strategy identified in the DSBSO crosslinked enrichable library with or without spiking using linear tryptic HEK peptides as indicated. Controls were directly used for measurement; enriched samples were subjected to affinity enrichment. Error bars indicate standard deviations of average values, experimentally validated FDR is shown as callout, n = 3 independent samples acquired on different days. Source data are provided as a Source Data file.