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. 2022 Jul 8;13:3972. doi: 10.1038/s41467-022-31735-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Expression of TRIM24 protein in various tissues of the 2.5-month-old female TRIM24^C52/55A and WT mice. B Subcellular distribution of endogenous TRIM24 in TRIM24^C52/55A and WT hepatocytes in response to insulin. After cellular fractionation, subcellular distribution of TRIM24 was measured in the nuclear and cytosolic fractions via immunoblotting. GAPDH was detected as a cytosolic marker, and Lamin A/C as a nuclear marker. Representative blots were shown here. Quantitation results were shown in Supplementary Fig. 8C. C Liver TG in 5-month-old male TRIM24^C52/55A and WT mice fed the CD. n = 5. D Liver TG in 5-month-old male TRIM24^C52/55A and WT mice fed the HFD. n = 7 (WT) and 9 (TRIM24^C52/55A). p = 0.0075. E KEGG pathway analysis of differentially-expressed genes in the liver of HFD-fed TRIM24^C52/55A and WT mice. F Expression of lipid metabolic genes in the liver of 5-month-old male TRIM24^C52/55A and WT mice on HFD. n = 7 (WT) and 8 (TRIM24^C52/55A). In case of Scd1 and Atgl, n = 7 for TRIM24^C52/55A. p = 0.025 (Pparγ), 0.0027 (Fasn), 0.018 (Acc1), 0.0062 (Scd1), 0.73 (Atgl) and 0.90 (Hsl). G, H. Protein levels of PPARγ, FASN, ACC, and SCD1 in the liver of 5-month-old male TRIM24^C52/55A and WT mice on HFD. G, representative blots. H Quantitation data. n = 6. p = 0.0057 (PPARγ), 0.000054 (FASN), 0.00056 (ACC) and 0.076 (SCD1). I, J LDs in TRIM24^C52/55A and WT primary hepatocytes transfected with empty vector or Flag-PPARγ. LDs in hepatocytes were stained with Bodipy. I Representative images. J Quantitation of the largest LDs in primary hepatocytes. Scale bars indicate 10 μm in length. n = 110 (WT/Vector), 120 (WT/Flag-PPARγ), 114 (TRIM24^C52/55A/Vector) and 127 (TRIM24^C52/55A/Flag-PPARγ). p = 0.0010 (WT/Vector vs WT/Flag-PPARγ), and p < 0.0001 for the rest. Data are given as the mean ± SEM. Statistical analyses were carried out via two-sided t-test for C, D, F, H, and via two-way ANOVA for J. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001. Source data are provided as a Source Data file.