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. 2022 Jul 8;13:3972. doi: 10.1038/s41467-022-31735-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Stability of Pparγ mRNA in TRIM24^C52/55A and WT hepatocytes upon treatment with actinomycin-D (ActD). n = 4. p = 0.0051 (TRIM24^C52/55A/6 h vs WT/6 h). B Stability of Pparγ mRNA in mouse primary hepatocytes expressing GFP-TRIM24 or free GFP upon ActD treatment. n = 3. p = 0.0496 (GFP-TRIM24/4 h vs GFP/4 h) and 0.016 (GFP-TRIM24/6 h vs GFP/6 h). C Pparγ mRNA levels in mouse primary hepatocytes expressing GFP-TRIM24 or free GFP. n = 3. p = 0.030. D-E. Pparγ mRNA in situ hybridisation in mouse primary hepatocytes expressing GFP-LSM1 (D) or GFP-TRIM24^S1043D (E). Scale bars indicate 10 μm in length. F Pparγ mRNA in RNA immunoprecipitates (RIP) from mouse primary hepatocytes expressing GFP-TRIM24^S1043D or GFP through the GFP antibody. n = 4. p = 0.017. G Pparγ mRNA in RIP from insulin-treated mouse primary hepatocytes using the TRIM24 antibody. n = 3. p = 0.0076. H Pparγ mRNA in RIP from HEK293 cells co-expressing Flag-EDC4 and GFP-TRIM24 or GFP using the Flag antibody. n = 3. p = 0.020 (Flag-EDC4/GFP vs Flag/GFP) and 0.012 (Flag-EDC4/GFP-TRIM24 vs Flag-EDC4/GFP). I Pparγ mRNA in RIP in HEK293 cells co-expressing Flag-AGO2 and GFP-TRIM24 or GFP using the Flag antibody. n = 3. p = 0.0005 (Flag-AGO2/GFP vs Flag/GFP) and 0.0001 (Flag-AGO2/GFP-TRIM24 vs Flag-AGO2/GFP). J, K Pparγ mRNA levels in the TRIM24^C52/55A and WT hepatocytes upon downregulation of Edc4 (J), or Ago2 (K). J n = 5 (TRIM24^WT/siNC, TRIM24^C52/55A/siNC, TRIM24^C52/55A/siEdc4) and 6 (TRIM24^WT/siEdc4) for Edc4. n = 5 (TRIM24^WT/siNC, TRIM24^WT/siEdc4) and 6 (TRIM24^C52/55A/siNC, TRIM24^C52/55A/siEdc4) for Pparγ. p < 0.0001. K n = 5 (TRIM24^WT/siNC, TRIM24^C52/55A/siNC, TRIM24^C52/55A/siAgo2) and 6 (TRIM24^WT/siAgo2) for Ago2. n = 6 for Pparγ. p = 0.0004 (TRIM24^C52/55A/siAgo2 vs TRIM24^C52/55A/siNC), and p < 0.0001 for the rest. L Ubiquitination of endogenous EDC4 in TRIM24^C52/55A and WT hepatocytes. Data are given as the mean ± SEM. Statistical analyses: two-sided t-test for A–C and F, G, one-way ANOVA for H, I, and two-way ANOVA for J, K. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001. Source data are provided as a Source Data file.