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. 2022 Jul 8;13(7):590. doi: 10.1038/s41419-022-05023-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Screening of the potential chemical agents able to downregulate NONO expression. THP-1 and MV4-11 cells were treated with the indicated concentrations of drugs, including DDP (1 μM), 5-FU (2 μM), paclitaxel (3.5 μM), MTX (0.1 μM), and ADM (0.1 μM), for 48 h or with UV radiation (30 J/m²), and the levels of the indicated proteins were evaluated by western blotting. DMSO was used as the negative control. B DDP and ADM downregulated the protein expression of NONO and SAMHD1 in AML cells. THP-1 and MV4-11 cells were treated with the indicated concentrations of drugs for 48 h, and the levels of the indicated proteins were evaluated by western blotting. C, D DDP and ADM sensitized AML cells to Ara-C. Plots of the CIs for Ara-C and DDP (C) or ADM (D) in THP-1 and MV4-11 cells, as determined using the Chou–Talalay method (n = 3). Red lines: AML cells were treated with DDP or ADM for 12 h and then added with a series concentration of Ara-C for 36 h. Black lines: Ara-C and DDP or ADM were added simultaneously into AML cells and then incubated for 48 h. For respective immunoblots, the protein levels were quantified by ImageJ software.