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. 2022 Jul 8;10(13):e15377. doi: 10.14814/phy2.15377

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© 2022 The Author. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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MOTS‐c antibody (1° Ab) an reactivity and specificity in control rat, mouse, rhesus macaque (Mk), and human skeletal muscle. MOTS‐c was identified at ~14 kDa following a 1‐h peptide block against a rat MOTS‐c peptide (top) and was expressed highest in the phenotypically slow mouse soleus (SOL) and almost undetectable in the fast mouse medial gastrocnemius (MG) muscle (middle). Serially diluted samples show specificity of the MOTS‐c antibody in human vastus lateralis (VL) and rodent (rat) SOL muscles (bottom). GAPDH protein was used as a loading control. PLT, plantaris; TA, tibialis anterior.