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HPLC analysis of CRISPR/Cas9-mediated mutant lines of ginseng roots. (A) Ginsenoside chromatograms of extracts from wild-type roots. (B-D) Ginsenoside chromatograms of three mutant lines (Cr4, Cr7, and Cr14) using sgRNA1 of P. ginseng. (E-F) Ginsenoside chromatograms of two mutant lines (Cr3 and Cr8) using sgRNA4. (G) HPLC chromatogram of ginsenoside standards.
