Fig. 5.

Genotyping of targeted induced mutant lines of ginseng roots induced by CRISPR/Cas9. (A) The figure presents mutations in individual alleles in Cr4, Cr7, and Cr14 mutants induced by sgRNA1 aligned to two corresponding wild-type fragments (PPTa and PPTg), as determined by Deep DNA sequencing. Target sequences of the sgRNA1 site are bold and in blue, and the protospacer adjacent motif (PAM) is underlined. Deleted nucleotides are shown with red hyphens, and inserted nucleotides are shown in red bold. The net change in length is noted to the right of each sequence (insertion and deletion). SNPs between PPTg and PPTa sequences are marked in dark red or green. (B) The figure presents mutations in individual alleles in Cr3 mutant induced by sgRNA4 aligned to two corresponding wild-type fragments (PPTa and PPTg), as determined by Sanger sequencing.