Fig. 2.

Effects of KRG on DEHP-induced NF-kB nuclear translocation and COX-2 expression in Ishikawa cells. (A) Ishikawa cells were pre-treated with KRG (100, 500 μg/mL) for 1 h and DEHP (10 μM) was treated 24 h. After incubation, cell viability was measured using MTT assay. (B) Ishikawa cells were pre-treated with KRG (100, 500 μg/mL) for 1 h and treated with DEHP (10 μM) for 24 h. The proteins indicated above were evaluated by Western blot analysis. (C) Ishikawa cells were pre-treated with KRG (100, 500 μg/mL) for 1 h and treated with DEHP (10 μM) for 8 h. COX-2 mRNA levels were confirmed by qRT-PCR. (D) Ishikawa cells were pre-treated with KRG (100, 500 μg/mL) for 1 h and treated with DEHP (10 μM) for 24 h. COX-2 and β-actin were evaluated by Western blot analysis. ∗p < 0.05, DEHP vs. DEHP + KRG.