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. Author manuscript; available in PMC: 2022 Jul 9.
Published in final edited form as: Anal Chem. 2019 May 15;91(11):6976–6980. doi: 10.1021/acs.analchem.9b01410

Figure 1.

Figure 1.

Incorporation of PTM analysis improves V2R protein coverage of key structural regions in the HDXMS analysis. (a) Without considering PTMs, the HDXMS heat map of V2R indicated that there is no sequence coverage between residues 340 and 360 (boxed) in the C-tail. (b) After the phosphopeptides were added to the peptide pool during HDXMS analysis, the sequence coverage for residues 340−360 (boxed) was obtained. The figure here demonstrates the improved protein sequence coverage. Enlarged heat maps with a detailed description are shown in Figure S1. (c) Sequences of two peptic phosphopeptides are indicated. Phosphorylation sites are highlighted in red.