FIG. 1.

PCR analysis of wildlife fecal sample DNA amplified with the SSU diagnostic primer set. PCR amplification products of DNA extracted from wildlife fecal samples and amplified with the SSU diagnostic primer set were visualized directly on agarose gels (A) and by Southern analysis with the SSU rDNA probe (B). Two template concentrations (1× and 2×) were used for each sample except sample 4250. The left and right lanes of each pair of lanes contained the high and low concentrations, respectively Lane +ve contained a PCR-positive control consisting of 2 pg of C. parvum GCH1 DNA. Lanes m contained size markers. The arrow indicates the expected 435-bp product.