FIG. 3.

PCR analysis of wildlife fecal samples amplified with the DHFR primer set. PCR amplification products of DNA extracted from wildlife fecal samples and amplified with the DHFR typing primer set were visualized directly on agarose gels (A) and by Southern analysis with the DHFR probe (B). Two template concentrations (1× and 0.1×) were used for each sample. The left and right lanes of each pair of lanes contained the high and low concentrations, respectively. Lane +ve contained a PCR positive control consisting of 2 pg of C. parvum GCH1 DNA. Lane −ve contained a no-template control. Lane m contained size markers, and lane b was empty. The arrow indicates the expected 232-bp product.