TABLE 1.

Primer sets used in this study to identify and characterize Cryptosporidium

Primer set	Target gene	Primer	Sequence	Expected product size (bp)	Annealing temp (°C)	Reference(s)
LSU	Large-subunit rRNA	LSU-F	5′-GTAACTGCGAGTGAACAGGAA-3′	280	60	This study
		LSU-R	5′-CCTCACGGTACTTGTTTGCT-3′
SSU	SSU rRNA	CPB-DIAGF	5′-AAGCTCGTAGTTGGATTTCTG-3′	435a	60	15
		CPB-DIAGR	5′-TAAGGTGCTGAAGGAGTAAGG-3′
DHFR	Dihydrofolate reductase-thymidylate synthase	DHFR1	5′-TTGTGGCAGCTTCTGTTTTGA-3′	232	68	This study
		DHFR2	5′-GGATCGGCTTCATCTTGTGG-3′			39
POLY(T)	Polythreonine-rich glycoprotein	CRY44	5′-CTCTTAATCCAATCATTACAAC-3′	318	52	3, 5
		CRY39	5′-GAGTCTAATAATAAACCACTG-3′
B-TUB	β-Tubulin	BTUB5	5′-GATTGGTGCTAAATTCTGGG-3′	478	60	4, 45
		BTUB4	5′-CCTGATCCTGTACCACCTCC-3′

^aCorresponding to position 601 to position 1035 of the C. parvum SSU rRNA sequence (GenBank accession number L16996).